mouse spleen Search Results


spleen cells  (ATCC)
94
ATCC spleen cells
Spleen Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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spleen dissociation kit  (Miltenyi Biotec)
96
Miltenyi Biotec spleen dissociation kit
Spleen Dissociation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse lymphocyte isolation kit  (Beijing Solarbio Science)
96
Beijing Solarbio Science mouse lymphocyte isolation kit
Mouse Lymphocyte Isolation Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse spleen mononuclear cell isolation kit  (Beijing Solarbio Science)
94
Beijing Solarbio Science mouse spleen mononuclear cell isolation kit
Mouse Spleen Mononuclear Cell Isolation Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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neutrophil mouse isolation kit  (Beijing Solarbio Science)
92
Beijing Solarbio Science neutrophil mouse isolation kit
Figure 4. Icaritin downregulates PD-L1 expression on MDSCs and <t>neutrophils</t> in the tumor microenvironment. C57BL/6 mice were treated with 70 mg/kg body weight of icaritin or vehicle control from day 3 to day 18 after the inoculation of B16F10 tumor cells. Tumors were digested into single cell suspensions and then subjected to surface staining. Percentage of Gr1+CD11b+ MDSCs in the tumor-infiltrating lymphocytes (A, B) and the surface expression level of PD-L1 on MDSCs (A, C, and D) from B16 tumors as compared to vehicle control were analyzed by flow cytometry (n = 4 in each group). Data are from one single experiment representative of two independent experiments. (E, F) Surface expression level of PD-L1 on neutrophils from PBMCs of icaritin-treated B16 mice as compared to vehicle control was analyzed by flow cytometry (n = 4 in each group). Data are from one single experiment representative of two independent experiments. (G, H) HL-60 cell lines were cultured with 1.25% DMSO for 6 days, TNF-α (G) and IL-6 (H) were used as positive controls for the induction of PD-L1 expression with or without the treatment of 20 µM icaritin for 24 h; PD-L1 expression level in the HL-60 cell lines was analyzed by western blot. Data are from one single experiment, representative of three independent experiments.
Neutrophil Mouse Isolation Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+spleen/Mouse+Spleen+Neutrophils+Isolation+Kit/pm31465113-159-6-10
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accession numbers atcc hb 12312  (ATCC)
90
ATCC accession numbers atcc hb 12312
Figure 4. Icaritin downregulates PD-L1 expression on MDSCs and <t>neutrophils</t> in the tumor microenvironment. C57BL/6 mice were treated with 70 mg/kg body weight of icaritin or vehicle control from day 3 to day 18 after the inoculation of B16F10 tumor cells. Tumors were digested into single cell suspensions and then subjected to surface staining. Percentage of Gr1+CD11b+ MDSCs in the tumor-infiltrating lymphocytes (A, B) and the surface expression level of PD-L1 on MDSCs (A, C, and D) from B16 tumors as compared to vehicle control were analyzed by flow cytometry (n = 4 in each group). Data are from one single experiment representative of two independent experiments. (E, F) Surface expression level of PD-L1 on neutrophils from PBMCs of icaritin-treated B16 mice as compared to vehicle control was analyzed by flow cytometry (n = 4 in each group). Data are from one single experiment representative of two independent experiments. (G, H) HL-60 cell lines were cultured with 1.25% DMSO for 6 days, TNF-α (G) and IL-6 (H) were used as positive controls for the induction of PD-L1 expression with or without the treatment of 20 µM icaritin for 24 h; PD-L1 expression level in the HL-60 cell lines was analyzed by western blot. Data are from one single experiment, representative of three independent experiments.
Accession Numbers Atcc Hb 12312, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse sp2 0 myeloma cells  (ATCC)
90
ATCC mouse sp2 0 myeloma cells
Figure 4. Icaritin downregulates PD-L1 expression on MDSCs and <t>neutrophils</t> in the tumor microenvironment. C57BL/6 mice were treated with 70 mg/kg body weight of icaritin or vehicle control from day 3 to day 18 after the inoculation of B16F10 tumor cells. Tumors were digested into single cell suspensions and then subjected to surface staining. Percentage of Gr1+CD11b+ MDSCs in the tumor-infiltrating lymphocytes (A, B) and the surface expression level of PD-L1 on MDSCs (A, C, and D) from B16 tumors as compared to vehicle control were analyzed by flow cytometry (n = 4 in each group). Data are from one single experiment representative of two independent experiments. (E, F) Surface expression level of PD-L1 on neutrophils from PBMCs of icaritin-treated B16 mice as compared to vehicle control was analyzed by flow cytometry (n = 4 in each group). Data are from one single experiment representative of two independent experiments. (G, H) HL-60 cell lines were cultured with 1.25% DMSO for 6 days, TNF-α (G) and IL-6 (H) were used as positive controls for the induction of PD-L1 expression with or without the treatment of 20 µM icaritin for 24 h; PD-L1 expression level in the HL-60 cell lines was analyzed by western blot. Data are from one single experiment, representative of three independent experiments.
Mouse Sp2 0 Myeloma Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+spleen/Hybridoma+--+fusion+of+mouse+spleen+cells+x+SP2%2F0%2C+14%2E16/pmc06723738-78-0-4
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mouse spleen extract  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology mouse spleen extract
Figure 4. Icaritin downregulates PD-L1 expression on MDSCs and <t>neutrophils</t> in the tumor microenvironment. C57BL/6 mice were treated with 70 mg/kg body weight of icaritin or vehicle control from day 3 to day 18 after the inoculation of B16F10 tumor cells. Tumors were digested into single cell suspensions and then subjected to surface staining. Percentage of Gr1+CD11b+ MDSCs in the tumor-infiltrating lymphocytes (A, B) and the surface expression level of PD-L1 on MDSCs (A, C, and D) from B16 tumors as compared to vehicle control were analyzed by flow cytometry (n = 4 in each group). Data are from one single experiment representative of two independent experiments. (E, F) Surface expression level of PD-L1 on neutrophils from PBMCs of icaritin-treated B16 mice as compared to vehicle control was analyzed by flow cytometry (n = 4 in each group). Data are from one single experiment representative of two independent experiments. (G, H) HL-60 cell lines were cultured with 1.25% DMSO for 6 days, TNF-α (G) and IL-6 (H) were used as positive controls for the induction of PD-L1 expression with or without the treatment of 20 µM icaritin for 24 h; PD-L1 expression level in the HL-60 cell lines was analyzed by western blot. Data are from one single experiment, representative of three independent experiments.
Mouse Spleen Extract, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse cat no 130 115 721  (Miltenyi Biotec)
92
Miltenyi Biotec mouse cat no 130 115 721
Figure 4. Icaritin downregulates PD-L1 expression on MDSCs and <t>neutrophils</t> in the tumor microenvironment. C57BL/6 mice were treated with 70 mg/kg body weight of icaritin or vehicle control from day 3 to day 18 after the inoculation of B16F10 tumor cells. Tumors were digested into single cell suspensions and then subjected to surface staining. Percentage of Gr1+CD11b+ MDSCs in the tumor-infiltrating lymphocytes (A, B) and the surface expression level of PD-L1 on MDSCs (A, C, and D) from B16 tumors as compared to vehicle control were analyzed by flow cytometry (n = 4 in each group). Data are from one single experiment representative of two independent experiments. (E, F) Surface expression level of PD-L1 on neutrophils from PBMCs of icaritin-treated B16 mice as compared to vehicle control was analyzed by flow cytometry (n = 4 in each group). Data are from one single experiment representative of two independent experiments. (G, H) HL-60 cell lines were cultured with 1.25% DMSO for 6 days, TNF-α (G) and IL-6 (H) were used as positive controls for the induction of PD-L1 expression with or without the treatment of 20 µM icaritin for 24 h; PD-L1 expression level in the HL-60 cell lines was analyzed by western blot. Data are from one single experiment, representative of three independent experiments.
Mouse Cat No 130 115 721, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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atcc pta 6123 71a1 abyssinia biologics n a 1c22 gift  (ATCC)
91
ATCC atcc pta 6123 71a1 abyssinia biologics n a 1c22 gift
Figure 4. Icaritin downregulates PD-L1 expression on MDSCs and <t>neutrophils</t> in the tumor microenvironment. C57BL/6 mice were treated with 70 mg/kg body weight of icaritin or vehicle control from day 3 to day 18 after the inoculation of B16F10 tumor cells. Tumors were digested into single cell suspensions and then subjected to surface staining. Percentage of Gr1+CD11b+ MDSCs in the tumor-infiltrating lymphocytes (A, B) and the surface expression level of PD-L1 on MDSCs (A, C, and D) from B16 tumors as compared to vehicle control were analyzed by flow cytometry (n = 4 in each group). Data are from one single experiment representative of two independent experiments. (E, F) Surface expression level of PD-L1 on neutrophils from PBMCs of icaritin-treated B16 mice as compared to vehicle control was analyzed by flow cytometry (n = 4 in each group). Data are from one single experiment representative of two independent experiments. (G, H) HL-60 cell lines were cultured with 1.25% DMSO for 6 days, TNF-α (G) and IL-6 (H) were used as positive controls for the induction of PD-L1 expression with or without the treatment of 20 µM icaritin for 24 h; PD-L1 expression level in the HL-60 cell lines was analyzed by western blot. Data are from one single experiment, representative of three independent experiments.
Atcc Pta 6123 71a1 Abyssinia Biologics N A 1c22 Gift, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+spleen/Hybridoma+from+fusion+of+SP2%2F0+with+A%2FJ+mouse+spleen%3A+266%2E2/pm37167969-128-50-49
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mouse spleen nk cells isolation kit  (Beijing Solarbio Science)
94
Beijing Solarbio Science mouse spleen nk cells isolation kit
Figure 4. Icaritin downregulates PD-L1 expression on MDSCs and <t>neutrophils</t> in the tumor microenvironment. C57BL/6 mice were treated with 70 mg/kg body weight of icaritin or vehicle control from day 3 to day 18 after the inoculation of B16F10 tumor cells. Tumors were digested into single cell suspensions and then subjected to surface staining. Percentage of Gr1+CD11b+ MDSCs in the tumor-infiltrating lymphocytes (A, B) and the surface expression level of PD-L1 on MDSCs (A, C, and D) from B16 tumors as compared to vehicle control were analyzed by flow cytometry (n = 4 in each group). Data are from one single experiment representative of two independent experiments. (E, F) Surface expression level of PD-L1 on neutrophils from PBMCs of icaritin-treated B16 mice as compared to vehicle control was analyzed by flow cytometry (n = 4 in each group). Data are from one single experiment representative of two independent experiments. (G, H) HL-60 cell lines were cultured with 1.25% DMSO for 6 days, TNF-α (G) and IL-6 (H) were used as positive controls for the induction of PD-L1 expression with or without the treatment of 20 µM icaritin for 24 h; PD-L1 expression level in the HL-60 cell lines was analyzed by western blot. Data are from one single experiment, representative of three independent experiments.
Mouse Spleen Nk Cells Isolation Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sp2 0 mouse myeloma cells  (ATCC)
90
ATCC sp2 0 mouse myeloma cells
Figure 4. Icaritin downregulates PD-L1 expression on MDSCs and <t>neutrophils</t> in the tumor microenvironment. C57BL/6 mice were treated with 70 mg/kg body weight of icaritin or vehicle control from day 3 to day 18 after the inoculation of B16F10 tumor cells. Tumors were digested into single cell suspensions and then subjected to surface staining. Percentage of Gr1+CD11b+ MDSCs in the tumor-infiltrating lymphocytes (A, B) and the surface expression level of PD-L1 on MDSCs (A, C, and D) from B16 tumors as compared to vehicle control were analyzed by flow cytometry (n = 4 in each group). Data are from one single experiment representative of two independent experiments. (E, F) Surface expression level of PD-L1 on neutrophils from PBMCs of icaritin-treated B16 mice as compared to vehicle control was analyzed by flow cytometry (n = 4 in each group). Data are from one single experiment representative of two independent experiments. (G, H) HL-60 cell lines were cultured with 1.25% DMSO for 6 days, TNF-α (G) and IL-6 (H) were used as positive controls for the induction of PD-L1 expression with or without the treatment of 20 µM icaritin for 24 h; PD-L1 expression level in the HL-60 cell lines was analyzed by western blot. Data are from one single experiment, representative of three independent experiments.
Sp2 0 Mouse Myeloma Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 4. Icaritin downregulates PD-L1 expression on MDSCs and neutrophils in the tumor microenvironment. C57BL/6 mice were treated with 70 mg/kg body weight of icaritin or vehicle control from day 3 to day 18 after the inoculation of B16F10 tumor cells. Tumors were digested into single cell suspensions and then subjected to surface staining. Percentage of Gr1+CD11b+ MDSCs in the tumor-infiltrating lymphocytes (A, B) and the surface expression level of PD-L1 on MDSCs (A, C, and D) from B16 tumors as compared to vehicle control were analyzed by flow cytometry (n = 4 in each group). Data are from one single experiment representative of two independent experiments. (E, F) Surface expression level of PD-L1 on neutrophils from PBMCs of icaritin-treated B16 mice as compared to vehicle control was analyzed by flow cytometry (n = 4 in each group). Data are from one single experiment representative of two independent experiments. (G, H) HL-60 cell lines were cultured with 1.25% DMSO for 6 days, TNF-α (G) and IL-6 (H) were used as positive controls for the induction of PD-L1 expression with or without the treatment of 20 µM icaritin for 24 h; PD-L1 expression level in the HL-60 cell lines was analyzed by western blot. Data are from one single experiment, representative of three independent experiments.

Journal: European journal of immunology

Article Title: Icaritin promotes tumor T-cell infiltration and induces antitumor immunity in mice.

doi: 10.1002/eji.201948225

Figure Lengend Snippet: Figure 4. Icaritin downregulates PD-L1 expression on MDSCs and neutrophils in the tumor microenvironment. C57BL/6 mice were treated with 70 mg/kg body weight of icaritin or vehicle control from day 3 to day 18 after the inoculation of B16F10 tumor cells. Tumors were digested into single cell suspensions and then subjected to surface staining. Percentage of Gr1+CD11b+ MDSCs in the tumor-infiltrating lymphocytes (A, B) and the surface expression level of PD-L1 on MDSCs (A, C, and D) from B16 tumors as compared to vehicle control were analyzed by flow cytometry (n = 4 in each group). Data are from one single experiment representative of two independent experiments. (E, F) Surface expression level of PD-L1 on neutrophils from PBMCs of icaritin-treated B16 mice as compared to vehicle control was analyzed by flow cytometry (n = 4 in each group). Data are from one single experiment representative of two independent experiments. (G, H) HL-60 cell lines were cultured with 1.25% DMSO for 6 days, TNF-α (G) and IL-6 (H) were used as positive controls for the induction of PD-L1 expression with or without the treatment of 20 µM icaritin for 24 h; PD-L1 expression level in the HL-60 cell lines was analyzed by western blot. Data are from one single experiment, representative of three independent experiments.

Article Snippet: Neutrophils from PBMCs were isolated by Neutrophil (mouse) Isolation Kit (Solarbio, China).

Techniques: Expressing, Control, Staining, Cytometry, Cell Culture, Western Blot